FastQCFastQC Report
Thu 8 Feb 2018
YD10_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD10_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36300351
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG118450.013.5850051
TGGGGTT246350.010.3742611
TAGCCAG311700.09.8115981
TAGGCCG25250.09.5503441
GGGGTTT306250.09.2852882
CGGGCGT70200.09.178245
TGGCCGG79350.09.1454611
TGGCCAG458100.09.1161571
TAGCCCG13350.09.1161021
TCCCGTT51100.08.9971711
TAGGGCG11800.08.9766131
AGGGGCG50700.08.9347991
AGGGTTT234250.08.8512561
AGGGCCG48950.08.8398561
TACTCGG144150.08.747066537
CTACTCG150050.08.46325936
TGGGGTC146650.08.4369781
TGGGGCG50500.08.434651
GTAGTCC242400.08.40287726
TGGCAAA206100.08.3215331