FastQCFastQC Report
Thu 8 Feb 2018
YD11_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD11_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56086485
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG185750.013.7227841
TAGGCCG39600.011.3826471
TGGGGTT376800.010.8202191
TAGCCAG486500.010.196371
TAGCCCG20900.010.0287091
TGGCGAA53100.09.8469531
AGGGTTT363800.09.8067841
GGGGTTT456450.09.7956742
TGGCCAG702150.09.3148511
TAGGGCG18650.09.3050841
TAGCCGA22300.09.1969741
TGGGCCG76050.09.0980341
AGGGGCG77950.08.9919241
TGGGGCG71150.08.9643741
CGGGCGT111850.08.8165765
TGGGGTC233800.08.7142961
TGGCCGG119300.08.6712281
AGCCGGG287600.08.5291552
TAGGCGG27100.08.4828011
TACTCGG226850.08.44737437