FastQCFastQC Report
Thu 8 Feb 2018
YD12_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD12_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60217658
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG178950.012.278681
TGGGGTT408600.010.1815081
TAGGCCG57600.09.9769191
TGGGCCG145350.09.9075131
TGGGGCG150600.09.8763791
TGGCCGG187250.09.6763811
AGGGGCG177350.09.6320081
TGGGGCC383850.09.3702271
AGGGCCG161700.09.2680941
AGGGTTT354800.09.2291311
TGGCGGC190500.09.15641
TAGCCAG442100.09.114351
GGGGTTT476150.09.0969612
TGGCCAG727400.08.9970911
AGGCGCC235150.08.97993851
AGGGGCC324800.08.8742841
AGGGCGG185750.08.8688511
TGGCGAA72050.08.7892351
TGGGCGG183050.08.778061
AGGCCGG270250.08.7225751
Produced by FastQC (version 0.11.5)