FastQCFastQC Report
Thu 8 Feb 2018
YD14_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD14_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19648201
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG67350.013.5500631
TATGCCG10750.011.10570845
TGGGGTT129750.09.9684681
TAGGCCG14250.09.8039421
TAGCCAG171250.09.7369971
TGGCGAA22700.09.6287471
AGGGTTT119100.09.2895281
TGATCGG10200.09.25783315
TGGGCCG34050.09.1324211
TGGCCGG48250.08.96661
TGGCCAG234300.08.9632921
TAGCCCG8100.08.9020251
CGGCTAA38850.08.74180120
TAGGCGC8250.08.7401711
AGGGGCG34950.08.63935851
TACTCGG79200.08.53467137
GGGGTTT152150.08.3960542
GATCGGA10750.08.3844671
CCGGCTA41150.08.361443519
CTACTCG84500.08.31933436
Produced by FastQC (version 0.11.5)