FastQCFastQC Report
Thu 8 Feb 2018
YD15_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD15_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51597875
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG145450.012.7209661
TGGGCCG118650.010.6178041
TGGGGTT334250.010.3766671
AGGGGCG142400.010.1288611
AGGGCGG154200.010.1283691
TAGGCCG44750.010.1226371
TGGGGCG124100.09.8791081
AGGGCCG126050.09.8156751
TGGCCGG149450.09.6208931
TGGCGGC158800.09.4943721
AGGGTTT317900.09.4783241
TGGCCCG88850.09.1060011
GGGGTTT394700.09.0907412
TAGCCAG380900.08.84546851
TGGCCAG616100.08.8010611
AGGCGCC180150.08.7569821
TGGGGCC310400.08.7562111
AGGGGGC238050.08.6625061
TGGGCGG148450.08.6381891
AGGCCGG220100.08.57031
Produced by FastQC (version 0.11.5)