FastQCFastQC Report
Thu 8 Feb 2018
YD16_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD16_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24677867
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC9200.012.4878645
TACGGCG9050.012.1970076
TGGGCCG81350.011.114691
TAGCCGG68650.010.4447281
TGGGGCG86250.010.2479611
TGGGGTT168550.09.9128831
AGGGCCG91250.09.8841121
AGGGCGG109200.09.8286721
AGGCCGG142800.09.7108551
AGGCGCC123750.09.69341851
TGGGCGG103300.09.6260651
TGATACG11300.09.5690443
AGGGGCG105900.09.5387751
TGGCCGG95250.09.4927031
TACACGA10250.09.43598438
TCGTATA9300.09.43110512
TGGCGGC113200.09.4216131
AGGCGGC122050.09.27421
GATACGG12500.09.010854
AGGCCCG92000.08.9702351
Produced by FastQC (version 0.11.5)