FastQCFastQC Report
Thu 8 Feb 2018
YD18_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD18_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19420366
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC7700.011.9938035
TACGGCG8550.011.8552346
TGATACG8300.011.669553
TGGCCGG75950.011.1028511
AGGGCCG66300.010.7804381
TAGCCGG58150.010.7404061
TAGGCCG20250.010.5776521
TGGGGCG64850.010.3261181
AGGGCGG83800.09.9551641
TGGGGTT130900.09.9386381
AGGCCGG113500.09.8134511
TGGCGGC91600.09.7720511
TGGGCCG60550.09.6816611
AGGGGCG80400.09.5628871
AGGCGGT32350.09.5485391
AGGCGCC91700.09.5155141
AGGGTTT113200.09.4211831
TAGCCAG131500.09.4131991
AGGGGGG53550.09.3472571
AGGGGGC119300.09.2985521
Produced by FastQC (version 0.11.5)