FastQCFastQC Report
Thu 8 Feb 2018
YD19_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD19_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58804314
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG181750.013.1283731
TGGGCCG145000.010.6697881
TAGGCCG53850.010.2607721
TGGCGAA70800.010.0340661
TGGGGCG153500.09.8291831
TGGGGTT397050.09.8265391
AGGGTTT361600.09.767041
AGGGCGG183000.09.7482151
AGGGGCG180950.09.67171
TGGGCGG181450.09.3964641
AGGGCCG162850.09.3617831
TGGCCGG181550.09.3167551
TAGCCAG444450.09.3164281
TGGCGGC190600.09.3121821
AGGCCGG262750.09.218521
TGGCCAG716950.09.0311691
TGGGGCC377200.08.9505511
AGGGGCC331750.08.9327271
TGGCGGA88600.08.8327391
TAGGCGC32000.08.809671