FastQCFastQC Report
Thu 8 Feb 2018
YD1_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD1_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48012841
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA21850.015.67538451
CTCGTAT22900.014.62904842
TCTCGTA25650.013.52726641
CGTATGC27650.012.3821644
ATCGGAA28500.012.0954462
TAGCCGG144000.010.5938111
TCGTATG32400.010.54784843
ACGGGGT121000.09.8141191
TGGGGTT297200.09.765471
TGGCGAA45200.09.5716961
ATCTCGT38700.09.25684240
TCCCGTT77950.09.1925721
ACACGTC37700.09.12463213
TCGGAAG39800.09.050793
CGGAAGA45650.08.7769594
TGGCCGG87300.08.7758811
GGGGTTT349400.08.6150712
CGGGGTT127250.08.4811162
AGGGCCG43400.08.3072321
GGCGAAA43800.08.2818442
Produced by FastQC (version 0.11.5)