FastQCFastQC Report
Thu 8 Feb 2018
YD20_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD20_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46268895
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG154400.013.9083121
TGGGGTT313050.010.253591
AGGGTTT292300.010.0322711
TAGCCAG405850.09.8321161
TAGCCCG19300.09.8176321
TAGGCCG30800.09.3744121
TGGCCAG578150.09.2117081
TGGGCCG66100.09.179851
TGGCCGG99500.09.1135531
TGGCGAA49250.09.0686721
GGGGTTT375250.08.9887052
CGGGCGT94250.08.9509895
AGGGGCG69950.08.9325771
TAGGGCG14900.08.7806451
TACTCGG191800.08.69326137
TAGCCGA21100.08.5524841
AGGGCCG63500.08.5255471
GTAGTCC327350.08.474133526
CTACTCG202450.08.4582436
CGGCTAA87550.08.35280420
Produced by FastQC (version 0.11.5)