FastQCFastQC Report
Thu 8 Feb 2018
YD21_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD21_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43281758
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG133150.012.7141711
TGGGCCG119650.010.6068151
TGGGGTT298250.010.4300881
AGGGCCG128700.010.2287751
TAGCCCG28950.010.1224141
TGGCGGC157400.010.0965661
AGGGGCG149700.010.0587481
AGGGCGG151400.09.9904711
TGGGGCG127200.09.9240791
TAGGCCG42050.09.7029071
TAGCCAG312850.09.6335111
AGGGTTT260550.09.3610711
AGGGGCC260600.09.2900731
TGGCCAG549100.09.2039341
AGGCGCG101900.09.114071
AGGCGCC181150.09.0341561
TGGGGCC303850.09.0211861
TGGCCGG148750.08.9712721
GGGGTTT358650.08.9584092
AGGCCGG215100.08.8867861
Produced by FastQC (version 0.11.5)