FastQCFastQC Report
Thu 8 Feb 2018
YD2_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD2_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35713686
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16700.016.733051
CTCGTAT18000.015.98767742
TCGTATG21400.013.756165543
TCTCGTA20700.013.62031641
CGTATGC21250.013.58011144
ATCGGAA21700.012.6670152
TAGCCGG122200.011.5813271
ACACGTC26950.011.08948713
ACGGGGT106550.010.4693961
ATCTCGT29450.010.03306240
TGGGGTT239350.09.5754111
CGTCTGA32450.09.14066616
CGGGGTT112150.08.9399462
TCGGAAG32950.08.8877533
TCCCGTT51800.08.8315341
TGGCCGG71800.08.6941121
GGCGAAA35300.08.6803722
TGGCGAA39500.08.6719431
AGGGTTT229150.08.6248111
AGGGGCG44150.08.5752831
Produced by FastQC (version 0.11.5)