FastQCFastQC Report
Thu 8 Feb 2018
YD3_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD3_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56989123
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22550.012.70673751
CTCGTAT25650.011.1390142
TAGCCGG168250.010.2450761
TCCCGTT98950.010.1922161
TCTCGTA29200.010.17000941
ACGGGGT149650.010.1012471
ATCGGAA29100.09.8977052
CGTATGC28100.09.68744744
TGGGGTT356200.09.3364221
CCAACGA103950.09.1763369
TCGTATG32450.09.08211243
TGGGCCG64600.09.045741
CGGGGTT160450.08.9193852
TCCAACG109500.08.8961428
ACGGAGT150150.08.8955591
CCCGTTT130050.08.8415432
TGGCCGG111500.08.7819661
TAGGCCG34550.08.7505151
TGGCCAG633150.08.7233181
CAACGAA112100.08.56939610
Produced by FastQC (version 0.11.5)