FastQCFastQC Report
Thu 8 Feb 2018
YD4_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD4_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46150537
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22750.012.9772861
CGTATGC23200.012.42720744
CTCGTAT23000.012.415194542
ATCGGAA25850.011.4144072
TCTCGTA25500.011.40485541
CGCCAAT27250.010.97147133
TAGCCGG147850.010.6549111
TCGTATG27150.010.51510543
TATCTCG30100.010.48581639
TAGGCCG31350.09.3454411
AGGCGCC129600.09.0773581
TAGCCGA19700.08.9232351
ACGGGGT137900.08.9068921
TGGCCGG107250.08.8046211
AGGGTTT282300.08.629961
AGGCCGG143500.08.6221281
ACGCCAA34650.08.5635232
TGGGGTT311900.08.4323651
AGGGGCG71400.08.3960961
ACGGAGT126350.08.3654941
Produced by FastQC (version 0.11.5)