FastQCFastQC Report
Thu 8 Feb 2018
YD5_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD5_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32733026
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18000.012.3944391
ATCGGAA18950.012.3652362
CTCGTAT17650.012.35554242
CGTATGC18800.011.5135344
TAGCCGG107400.011.4774971
TCTCGTA19150.011.30888941
ACGGGGT103750.010.7952431
TCGTATG20100.010.6204643
TCCCGTT48150.010.2965221
TGGGGTT219550.09.545831
CGGGGTT112650.09.0403572
AGGGCCG66450.08.7835261
TAGGCCG25850.08.7177331
TGGCCGG92900.08.6357181
CCAACGA43200.08.5300589
TGGGCCG62700.08.4822031
TAGCCAG270950.08.4668681
TGGCCAG401150.08.465851
GGGGTTT264600.08.3362592
AGGGTTT198700.08.3132391
Produced by FastQC (version 0.11.5)