FastQCFastQC Report
Thu 8 Feb 2018
YD6_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD6_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50667183
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG74100.010.6243051
TGGCCGG49450.09.2146391
AGGGGCG37450.09.1555611
TCCCGTT113400.09.0111121
TGGGGTT251100.08.9026681
AGGGCGG38150.08.57366851
AGGGCCG34600.08.5406021
CCAACGA123400.08.513429
TGGCCAG384750.08.4895221
TGGGGCG32850.08.4462321
TCCAACG128050.08.415088
TAGGCGT21450.08.4130971
TAGGCCG23050.08.3184281
GGGGTTT270400.08.2247162
CCCGTTT140050.08.2214482
TAGCCGA14300.08.202771
TGGCGAA32200.08.1964021
TGGCGGT24800.08.1862331
TAGCCAG275150.08.1819021
TGGGCCG31250.08.0846511