FastQCFastQC Report
Thu 8 Feb 2018
YD8_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD8_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35192113
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG76400.010.6817021
AGGGCCG125550.010.3785411
AGGGGCG155400.010.2998891
TGGCGGC168050.010.289211
TGGGGCG126200.09.9499531
AGGGCGG160950.09.734621
TGGGCCG116150.09.6075171
AGGGGGC216100.09.3471341
TGGGCGG133900.09.3104311
AGGGGCC237150.09.2304161
AGGCGCC160150.09.065341
TGGCCGG127850.09.0633261
AGGGGGG104800.09.05621
TGGGGCC258600.09.0227111
AGGGTTT199750.08.9497641
AGGGCGC100550.08.9457351
TAGGCCG35550.08.8145551
TGGGGTT231450.08.7174831
TAGGGCG23600.08.69269851
TGGGGCT256050.08.5050631
Produced by FastQC (version 0.11.5)