FastQCFastQC Report
Thu 8 Feb 2018
YD9_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD9_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27053104
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG60950.012.497341
TGGGGCG98650.011.1023211
AGGGGCG115850.010.6600481
TGGGCCG84450.010.6475041
TAGGCCG26850.010.4915661
AGGGCCG91900.010.4464481
TGGGGTT176600.010.2470641
TGGCGGC118750.010.1719871
AGGGCGG115400.09.9009481
TAGCCAG155850.09.8183281
CATACGC11550.09.739511517
ATACGCC11400.09.47428718
TGGCGAC35100.09.4381051
TCATACG11000.09.40836816
TGGGCGG105250.09.3997651
AGGGGGC166900.09.2222951
AGGGGGG82450.09.1291511
TGGCGCC74800.09.128841
TAGGCGT15850.09.0996631
TGGCCGG98400.09.092221
Produced by FastQC (version 0.11.5)