FastQCFastQC Report
Thu 8 Feb 2018
OD10_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD10_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55762890
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG101850.011.8026481
TGGGCCG54050.010.7870651
TGGGGCG51100.010.6168431
TCGTATA41050.010.46900912
AGGCCGG112100.010.3218311
TGGGGTT331600.010.2848351
TCCCGTT121250.010.2112791
TGGCCGG85250.09.8230821
CTTCGTA50900.09.81353810
AGGGCCG52800.09.7207581
AGGGGCG52950.09.6932211
TGGCCAG524550.09.5786991
TCCAACG138150.09.4625788
CCAACGA135450.09.4518989
GGGGTTT363300.09.4259022
ATCTTCG63150.09.3705838
ACTAGAC157700.09.3166122
TAGGCGA15250.09.2997221
AGGGCGG56300.09.2763861
TAGGCCG26950.09.1882571
Produced by FastQC (version 0.11.5)