FastQCFastQC Report
Thu 8 Feb 2018
OD12_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD12_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54792741
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG101750.011.5740361
TGGGGTT320300.010.3412551
TCGTATA41300.010.18774412
TGGGCCG57600.010.0077161
AGGGCGG61550.09.9508091
TCCCGTT117850.09.8973221
TGGGGCG55950.09.7394131
TGGCCGG90700.09.6822181
TGGCCAG515500.09.2821331
TGGGGGC234900.09.2600141
AGGGTTT311300.09.2441991
CTTCGTA52600.09.196710
GGGGTTT344700.09.1904082
TAGGCCG29400.09.0375811
CCAACGA125150.09.00717459
AGGGGCG61850.08.919571
CGTATAA46700.08.86512113
TCCAACG130950.08.8488298
TGGGCCA228700.08.831691
TGGGGCC222950.08.827171
Produced by FastQC (version 0.11.5)