FastQCFastQC Report
Thu 8 Feb 2018
OD13_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD13_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51601696
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG109950.011.9608191
TAGGCCG25850.010.7148941
TGGGGTT316400.09.9924961
TGGGCCG68750.09.4332881
TGGGGCG78050.09.2325221
TAGCCGA20200.09.1412541
TGGCCGG106150.09.0159651
AGGGCGG89300.08.9520031
TAGCCAG332800.08.9316961
GGGGTTT345050.08.8356932
AGGGTTT294500.08.8239661
TCCCGTT78750.08.8073141
AGGGCCG69550.08.7743611
TGGCGAA46800.08.6610381
TGGGCGG87150.08.4751971
TGGCCAG526950.08.4186051
TGGGGGC274850.08.3569491
AGGGTCG33750.08.340261
AGGGCGC62650.08.3389281
AGGCGCC114750.08.3206351
Produced by FastQC (version 0.11.5)