FastQCFastQC Report
Thu 8 Feb 2018
OD14_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD14_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53004448
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG118900.012.2368091
TAGGCCG32550.010.4482471
TGGGGTT327150.09.5762991
AGGGGCG97050.09.5381411
TGGGCCG81350.09.4686071
TGGCCGG119300.09.1751531
GGGGTTT357150.08.9581272
AGGGCCG80650.08.936411
TGGCCAG560550.08.8756241
TAGGCGC21700.08.7184061
TAGCCAG348050.08.7035851
AGGGTTT306900.08.6963881
AGGGGGC215700.08.6143261
TGGCAAA306050.08.5292051
TATGCCG20050.08.41610345
TGGGTTT311300.08.4070661
TGGGGCT323550.08.36720851
TGGGGCG90500.08.3619621
TGGGGGT282400.08.3582261
TAGCCCG21650.08.3224191