FastQCFastQC Report
Thu 8 Feb 2018
OD15_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD15_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51009857
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG110500.012.086791
TAGGCCG27200.010.6816761
TATGCCG20250.010.33298841
TGGGGCG83600.09.8873151
TGGGGTT326350.09.7516281
AGGGTTT294600.09.5870151
GGGGTTT348250.09.1612062
TGGCCGG107950.09.1592621
AGGGCCG79150.08.9635131
TGGGCCG81650.08.9373231
TAGGGCG18350.08.8372061
AGGGGCG95000.08.795671
TGGCCAG536650.08.7924821
AGGGGGG115050.08.7506331
TGGGGCC257450.08.6258641
TCCCGTT75900.08.5758011
TAGCCGA21300.08.5649341
AGGGGGC201250.08.5501961
TGGGCGG103650.08.5396841
TGGGGGC288700.08.52691651
Produced by FastQC (version 0.11.5)