FastQCFastQC Report
Thu 8 Feb 2018
OD16_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD16_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21549437
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG139500.022.9279691
AGGGGGG62800.017.527651
GGGGGGG81500.015.8020622
TGGGGGC141200.015.7828631
GGGGGGT66400.014.4363082
TGGGGGA150200.014.2364581
GGGGGGA83400.014.1980442
TGGGGGT126450.013.770891
GGGGGGC82500.013.5601032
TGGGGCG29200.013.3637031
TAGCCGG58800.013.3111531
AGGGGGC92300.012.9520261
TCCCCCC78250.012.7697661
TGGGGTT143750.011.6585491
TGGGGCA119250.011.3111391
TAGGGCG6350.011.0116281
AGGGGGT70350.010.9013021
TGGGGCT135750.010.8335631
GGGGGCT110500.010.7159892
GGGGGCC84450.010.6830582
Produced by FastQC (version 0.11.5)