FastQCFastQC Report
Thu 8 Feb 2018
OD17_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD17_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20759471
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG142650.020.8688891
AGGGGGG68100.016.2272951
TGGGGGC148300.015.2682671
GGGGGGG87650.014.4597432
TCCCCCC78650.013.7064921
TGGGGGT128300.013.2538431
TGGGGGA142650.012.679431
GGGGGGC90600.012.6447932
TGGGGCG40050.012.6137051
GGGGGGT69550.012.6132972
GGGGGGA88500.012.2058612
AGGGGGC93900.011.9368011
AGGGGGT70300.011.8056441
TGGGGCT137500.011.7109641
AGGGGCG39950.011.5726881
TGGGGCA113900.011.4644171
TGGGCCG30850.010.9656211
TGGGGTT138250.010.6360311
GGGGGCT107800.010.6272552
TGGGCCA103500.010.480981
Produced by FastQC (version 0.11.5)