FastQCFastQC Report
Thu 8 Feb 2018
OD18_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD18_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17581992
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG133050.023.2405511
AGGGGGG57650.017.4876731
TGGGGGC133200.017.2880231
GGGGGGG79600.016.2064632
GGGGGGT60250.014.7858162
TGGGGGT114650.014.6950261
TGGGGCG31250.014.1458761
GGGGGGA78050.013.7543272
GGGGGGC79550.013.5516752
TGGGGGA131000.013.4979751
TCCCCCC71550.012.9870781
AGGGGGC86600.012.7354651
AGGGGGT66800.012.5937831
TGGGGCA101700.012.2638841
TGGGGCT119150.011.9631851
TAGCCGG44050.011.77621651
TGGGCCA92550.011.6973821
AGGGGCG31450.011.5459341
GGGGGCT101950.011.5032722
TGGGGTT119500.011.3996851
Produced by FastQC (version 0.11.5)