FastQCFastQC Report
Thu 8 Feb 2018
OD19_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD19_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25299898
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19200.023.66526842
CGTATGC20250.022.88173144
GATCGGA21000.022.6808621
TGGGGGG185950.021.7296751
TCTCGTA21150.021.37700841
TCGTATG21500.021.23754143
ATCGGAA22950.020.7499432
AGGGGGG93900.019.4722351
ATCTCGT26500.018.07985740
ACACGTC28750.016.5062813
TGGGGGC198650.015.48829651
CGTCTGA32100.014.85284616
TCGGAAG33000.014.7030993
AGGGGGC138000.014.1328921
GTATGCC34900.013.98960645
CGGAAGA36900.013.7002214
TCCCCCC118300.013.6623261
GGGGGGA113600.013.1322832
AGGGGGT94400.012.9366441
TGGGGCG52500.012.6410861
Produced by FastQC (version 0.11.5)