FastQCFastQC Report
Thu 8 Feb 2018
OD1_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD1_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58893954
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG125600.012.37120151
TGGGGTT366250.010.0652251
TAGGCCG31500.09.7225631
AGGGGCG93050.09.4868521
AGGGTTT345050.09.1629961
GGGGTTT401500.09.0895392
TGGGGCG89800.08.8772681
TGGCCGG120700.08.86214351
TAGCCAG387300.08.7855591
TGGGCCG85150.08.7802291
TGGGGCC298650.08.7467741
AGGCGCC136650.08.45395851
AGGGCGG101100.08.3973571
AGGGGGC222300.08.3269271
TGGGGGC315450.08.3237771
TGGCCAG600350.08.1997011
TCCCGTT81200.08.1812261
TGGCAAA337450.08.161491
TGGCAAC166500.08.1555921
AGGGCCG82800.08.1047261
Produced by FastQC (version 0.11.5)