FastQCFastQC Report
Thu 8 Feb 2018
OD20_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD20_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22448324
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG151300.022.0494671
AGGGGGG78550.019.1664731
TGGGGGC155200.015.9979211
AGGGGGC106400.014.4891081
TCCCCCC90650.014.4667461
GGGGGGT75900.013.9453232
TGGGGGT133000.013.5769081
TGGGGCG38250.013.5724741
GGGGGGA94300.013.282472
TGGGGGA153950.012.7996211
GGGGGGC98600.012.6345462
AGGGGGT76550.012.6200521
TGGGCCG32900.012.349211
GGGGGGG131100.012.2739522
TGGGGCT143000.012.0434541
TAGCCGG47550.011.6774271
AGGGGGA113500.011.3951841
TGGGGCA129900.011.3813731
AGGGGCG41650.011.3264511
TGGGGCC124650.011.2269541
Produced by FastQC (version 0.11.5)