FastQCFastQC Report
Thu 8 Feb 2018
OD2_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD2_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60865398
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25550.011.1915361
CTCGTAT26500.010.69791842
TATCTCG29800.010.117331539
CGTATGC29650.09.86491244
TAGCCGG145300.09.6383281
ACGGGGT141700.09.2952891
TCTCGTA31300.09.2011141
TCGTATG32300.08.84658743
ATCGGAA32000.08.7889892
TGGGGTT366400.08.7627611
TAGGCCG32900.08.6912991
AGGGGCG92600.08.4857711
TGGCCGG120100.08.1362371
AGGGTTT350950.08.0386421
GGGGTTT409550.07.89457372
CGGGGTT151900.07.85049342
TGGCGAA57800.07.4791161
GCGTGAG133550.07.4466053
TGGCGTT45550.07.41446451
TGGCAAA351000.07.3254761
Produced by FastQC (version 0.11.5)