FastQCFastQC Report
Thu 8 Feb 2018
OD3_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD3_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61542064
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG139650.011.1467721
TAGGCCG34900.010.1986731
TGGGGTT402100.09.9107321
TGGGCCG111650.09.6647091
AGGGCGG139300.09.3958711
TGGGGCG123900.09.3637091
GGGGTTT437300.09.1683962
AGGGTTT351150.09.16109851
AGGCGCC176550.09.1232661
TGGCCAG679350.09.1124511
AGGCCGG195150.09.0040531
AGGGGCG137300.08.9092531
AGGGCCG115250.08.7959661
AGGGGGC288150.08.7482961
TGGGGCC353500.08.7242091
TGGGGGC395100.08.6894031
AGGGGGG156550.08.6483531
TGGCCGG160100.08.6395091
TGGGGCA339800.08.4527651
TAGCCAG385850.08.3722591
Produced by FastQC (version 0.11.5)