FastQCFastQC Report
Thu 8 Feb 2018
OD4_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD4_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55341521
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG123400.010.4931851
TAGGCCG34050.09.7881191
TGGGCCG91300.09.7427371
AGGGTTT315050.09.5209311
TGGGGTT338600.09.31098651
AGGGGCG107550.09.2757271
AGGGCGG115800.09.1399531
TGGCCAG583650.09.0902831
TGGGGCG97550.09.00309851
TGGCCGG136800.08.9550381
AGGGCCG93950.08.8447231
AGGCGCC150150.08.6987591
TGGGGCC290150.08.6693241
AGGCCGG165350.08.5936921
AGGGGGG121650.08.5708431
TGGGGGC314100.08.560341
TCCCGTT96650.08.5277391
AGGGGGC227150.08.5159891
TCGTATA32150.08.46778112
GGGGTTT369600.08.4435422
Produced by FastQC (version 0.11.5)