FastQCFastQC Report
Thu 8 Feb 2018
OD5_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD5_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57772206
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG119900.011.3050391
TGGGGTT345000.09.8222781
TCCCGTT92500.09.3716011
TGGGGCG79600.09.2497391
TAGGCCG32050.09.2031821
GGGGTTT367600.08.9117022
TGGCCGG111200.08.8282881
AGGGTTT329500.08.77413751
TAGCCAG377200.08.7509881
TGGGGGC287600.08.6510371
TAGCCCG22300.08.5824021
AGGGGCG82000.08.5671331
TGGGCCG70050.08.5500421
TGGCCAG579100.08.522791
AGGGCCG74250.08.4606271
AGGCGCC130200.08.33548451
TGGGGCA283950.08.2388861
AGGGCGG92500.08.2275351
AGGCCGG142800.08.0257261
TAGGCCA124350.08.0214471
Produced by FastQC (version 0.11.5)