FastQCFastQC Report
Thu 8 Feb 2018
OD7_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD7_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49312953
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG108700.011.8491251
TAGGCCG26200.011.258741
TGGGCCG58850.010.2160871
TCCCGTT93400.010.1738511
TGGGGTT302400.09.9109691
TGGGGCG61950.09.4140881
AGGCCGG122650.09.1979271
GGGGTTT333550.09.1266392
AGGGCCG62850.09.1001431
TGGCCGG94750.09.0782881
TGGCCAG516000.09.0724511
AGGGTTT284600.08.9959151
AGGGGCG67950.08.8810671
TGGCGAA47750.08.8655211
TGGGGGC230300.08.8192651
TGGGGCC224850.08.7125671
TAGCCCG17100.08.6909571
TAGCCAG325800.08.5010751
TGGCAAA288450.08.4308821
TGGGGTC189550.08.3987651
Produced by FastQC (version 0.11.5)