FastQCFastQC Report
Thu 8 Feb 2018
OD9_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD9_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58885326
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG134200.012.1486891
TCCCGTT115650.09.8525411
TGGGGTT327750.09.8250941
TGGCGAA46700.09.54754451
TGGCCAG549050.09.2650491
TAGCCCG18300.09.2289771
GGGGTTT359550.09.1549692
TAGCCAG418600.09.1505771
TGGGCCG47600.09.1304831
TAGGCCG29150.09.1156331
AGGGTTT335550.08.8719191
TGGCCGG80250.08.8391171
CCAACGA127900.08.7079549
TACTCGG159700.08.678575537
TCCAACG132650.08.5657988
TGGGGCG41600.08.4445141
CGGGCGT74150.08.4354325
CTACTCG168700.08.42897936
AGGGGCG45700.08.4260361
TGGCAAA356300.08.3931631
Produced by FastQC (version 0.11.5)