FastQCFastQC Report
Thu 8 Feb 2018
YD10_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD10_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52627479
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG120700.011.0816561
TGGGGTT302950.08.8822391
TCCCGTT96700.08.5460431
TAGGCCG24000.08.5379911
TGGCGAA40500.08.5067161
TGGCCAG503600.08.4911161
AGGGTTT306900.08.4891061
TACTCGG140450.08.29810537
TAGCCAG386500.08.2380691
GGCGAAA37550.08.148912
TGGGCGG52900.08.0025371
TGGCCGG67250.07.96911671
GGGGTTT337400.07.9287972
CTACTCG146450.07.835244736
CGGGCGT65350.07.8154745
TCGAACT134250.07.776454415
CTCGGGA154300.07.771923539
TGGCAAA314200.07.76153851
CGAACTC127350.07.703099316
ACCGCGC29250.07.615543412
Produced by FastQC (version 0.11.5)