FastQCFastQC Report
Thu 8 Feb 2018
YD11_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD11_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56218620
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG129250.012.8716841
TAGGCCG29950.011.8011491
TGGGGCG43650.010.5728261
TGGCCGG81350.010.4882531
TGGGGTT328950.010.4161261
TGGGCCG46350.09.7140811
ACCGCGC38650.09.60546312
CGGGCGT68400.09.4409625
TGGCCAG556100.09.4405461
GGGGTTT372750.09.367982
AGGGTTT337800.09.31018451
TGGCCGA37300.09.1135921
GCGTGAG113350.08.991843
TCCCGTT102800.08.9786741
TAGCCAG395850.08.8377531
TGGCAAA348550.08.8098951
AGGGGCG46150.08.731781
TGGGGGG166150.08.7122861
AGGGCCG42900.08.7110851
CCGGGCG116350.08.6443114