FastQCFastQC Report
Thu 8 Feb 2018
YD15_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD15_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54913841
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG136950.010.9000931
TGGGGTT324950.09.4024721
TGGCCGG97000.08.9597171
TGGGCCG57200.08.89592551
GGGGTTT362400.08.7478892
TGGCCAG555700.08.6179871
TAGCCGA20750.08.3550941
TGGCAAA323900.08.1400051
TGGCGAA49250.08.1375281
AGGGTTT315800.08.0208261
TAGCCAG406550.07.90294031
GGCGAAA48650.07.72349932
TACTCGG168750.07.719914437
TCCCGTT89150.07.6271861
AGGGCCG58200.07.62117531
AGGCCGG120850.07.52685261
CTACTCG173400.07.512912836
AGGGCGG69400.07.46185971
TAGGCCG30250.07.368661
TGGGGGC248200.07.35694931