FastQCFastQC Report
Thu 8 Feb 2018
YD16_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD16_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23147199
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG153700.023.113121
AGGGGGG66200.016.8995651
TGGGGGC147650.016.2692681
GGGGGGG90850.015.5657482
TGGGGGA160850.015.1585081
GGGGGGT75050.015.0561672
TGGGGGT133550.014.6428981
TCCCCCC88200.013.5793121
GGGGGGC87500.013.3263092
GGGGGGA90800.013.2642532
AGGGGGC93900.012.8751131
TGGGGCG37050.012.7844751
TAGCCGG53000.011.7033081
TGGGGTT148000.011.5977771
AGGGGGT74550.011.5273421
TGGGGTC99000.011.3460841
GGGGGCT111600.011.3175182
GGGGGTT81900.011.2082522
AGGGGGA112250.011.011491
AGGGCCG29550.010.9914971
Produced by FastQC (version 0.11.5)