FastQCFastQC Report
Thu 8 Feb 2018
YD17_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD17_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58329526
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG152450.012.7237831
TGGGGTT370200.09.9560581
TAGGCCG32350.09.6104851
AGGGTTT341800.09.5441451
AGGGCCG81200.09.4332951
TAGCCAG434650.09.215791
TGGGCCG79800.09.1470831
TGGCCGG122800.09.1179781
GGGGTTT403450.09.0621022
TGGCCAG636300.08.9435911
TAGCCCG26600.08.5542171
AGGGGCG94850.08.4795221
TGGCGAA60000.08.3732521
TACTCGG176550.08.33446937
TGGGCCA274100.08.2603331
TGGGGCG83850.08.2485161
GGCGAAA56700.08.2139612
TGGGGGC310550.08.096051
TAGGCGG27000.08.0937261
TGGCAAA335550.08.0568361
Produced by FastQC (version 0.11.5)