FastQCFastQC Report
Thu 8 Feb 2018
YD18_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD18_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20608970
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG139500.022.5560591
AGGGGGG66700.019.8987671
TGGGGGC136650.015.3289721
TCCCCCC82600.014.1008191
GGGGGGA87100.014.0440162
TAGCCGG58650.013.9705421
GGGGGGT67000.013.8781812
TGGGGCG32200.013.6695241
AGGGGGC94700.013.6577551
TGGGGGT120150.013.2446371
GGGGGGC80700.013.1721772
GGGGGGG113200.013.078792
TGGGGGA142600.013.0273171
TGGGGTT135650.011.6314011
AGGGGGA104350.011.3780531
TGGGGCA112650.011.1809231
AGGGGGT69300.011.1721191
TGGGGCC109350.010.7752271
AGGGGCG32250.010.6386991
GGGGGTT67350.010.6226242
Produced by FastQC (version 0.11.5)