FastQCFastQC Report
Thu 8 Feb 2018
YD19_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD19_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21407912
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG155450.021.5578921
AGGGGGG69400.017.3199231
TGGGGGC156650.016.65641
GGGGGGG92150.015.4903832
TGGGGGT135600.013.537641
GGGGGGT73400.013.4564922
TGGGGGA159650.013.1933821
AGGGGGC100650.013.017891
GGGGGGA92400.013.00795652
TGGGGCG42050.012.8177011
GGGGGGC99400.012.4095132
AGGGGGT78000.012.3166681
AGGGCCG35350.012.2487011
TCCCCCC92650.012.0486391
TGGGCCG37000.011.9462771
TGGGGCA128450.011.780581
TGGGGCT149700.011.6750341
TAGCCGG48850.011.4951071
AGGGGCG41150.011.399131
TGGGGTT145400.011.3533741
Produced by FastQC (version 0.11.5)