FastQCFastQC Report
Thu 8 Feb 2018
YD1_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD1_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59733257
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG160700.012.7500761
TGGCGAA51000.010.1983311
TGGGGTT347500.09.9652631
TAGCCAG480500.09.5498841
GGCGAAA50300.09.5277692
TAGCCCG20050.09.0961571
TGGCCAG598950.08.9882741
GGGGTTT395250.08.9259372
AGGGTTT347900.08.7953341
TCCCGTT101450.08.7666231
TACTCGG189700.08.68211637
AGCCGGG227550.08.543122
CTACTCG197250.08.41828636
GTAGTCC352350.08.39696626
CGGGCGT85700.08.1388445
TGGCAAA358000.08.0943661
TGGCCGG89000.08.0702691
CTCGGGA205550.08.01254839
CGCCTGT302250.08.00230121
TAGGCGT26600.07.87206361
Produced by FastQC (version 0.11.5)