FastQCFastQC Report
Thu 8 Feb 2018
YD20_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD20_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21826855
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG158350.022.391871
AGGGGGG63800.018.6314141
GGGGGGG85700.017.4226462
TGGGGGC160000.017.128221
TGGGGCG35500.016.2655031
TCCCCCC84200.015.028191
TGGGGGT143450.014.528721
TGGGGGA157150.014.5108431
GGGGGGT73500.014.42272952
AGGGGGC98450.014.2046941
TGGGCCG32200.013.7295441
GGGGGGC89750.013.6207732
GGGGGGA88600.013.5429992
AGGGGGT73950.013.359551
AGGGGCG34200.012.9925991
GGGGGTT80300.012.6677062
TGGGGCA124200.012.6035771
TAGGCCG12600.012.3519191
AGGGCCG32650.012.2277341
TGGGGCT147800.012.1934971