FastQCFastQC Report
Thu 8 Feb 2018
YD21_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD21_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53545604
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG121150.011.2688891
TAGGCCG32400.010.3603211
TGGGGCG110400.09.9378371
AGGGCCG100150.09.7177291
TGGGGTT341100.09.5965651
AGGGGCG122900.09.47699451
AGGGTTT305150.09.0512611
TGGGCCG99250.09.0340921
TGGCCGG136300.09.0080871
TAGGGCG22050.08.9909591
AGGCGCC159450.08.9294461
TGGGGCC309100.08.78253651
TGGCCAG573600.08.7348941
TGGCGAA59400.08.6852241
GGGGTTT371900.08.6511632
AGGGGCC269200.08.63647651
AGGGGGC251250.08.5720311
AGGGCGG131600.08.42250251
TAGCCAG338750.08.412851
TAGGCGT28000.08.36772251
Produced by FastQC (version 0.11.5)