FastQCFastQC Report
Thu 8 Feb 2018
YD2_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD2_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60747670
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG150800.013.3825611
TGGGGTT346850.09.883381
TAGCCAG460650.09.34865951
TAGCCGA23000.09.1072591
CGGGCGT83100.08.9076215
GGGGTTT394700.08.8982112
TCCCGTT97400.08.7642111
TACTCGG190000.08.751224537
CTACTCG191100.08.72442836
AGGGTTT356200.08.7070811
AGCCGGG221900.08.6589952
TAGCCCG18500.08.6440891
TGGCCAG618900.08.5849941
GTAGTCC344400.08.28394726
TGGCGAA51100.08.2423921
CGGCTAA75800.08.22216920
CTCGGGA204300.08.12761439
GCCGGGC263650.08.1241153
AGGCGCC95700.08.0726241
GGCGCCC61600.08.071952
Produced by FastQC (version 0.11.5)