FastQCFastQC Report
Thu 8 Feb 2018
YD3_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD3_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78568192
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29950.012.921020541
CTCGTAT32450.011.64860337
TAGCCGG175050.011.6290351
CGTATGC35800.010.87278939
TCTCGTA37750.010.37078436
TCGTATG42850.08.92642138
TGGGGTT434750.08.8985841
ACACGTC50350.08.6243058
TAGCCAG553400.08.3986191
TAGGCGT35450.08.3848461
ATGCCGT48650.08.37067342
CGTCTGA55200.08.15197311
TGGCCAG738400.08.1424841
AGGGTTT448200.08.058791
TCCCGTT139500.07.87740141
TACTCGG215450.07.780169537
GGGGTTT494350.07.7372522
AGCCGGG249350.07.6246462
CTACTCG222650.07.619526436
CGAACTC203000.07.55893716
Produced by FastQC (version 0.11.5)