FastQCFastQC Report
Thu 8 Feb 2018
YD4_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD4_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78064902
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG251450.013.4063571
TAGCCAG696300.010.2324751
TGGGGTT494300.010.0725351
TGGCCAG890650.09.8857661
TAGCCGA33800.09.7935751
TGGGCCG86650.09.4596151
TGGCCGG149950.09.3558271
GGGGTTT579100.09.30135252
TGGCGAA77150.09.1650391
AGGGTTT467300.09.1317511
TAGGCCG47000.09.1032481
TGGCAAA456450.08.9936111
TCCCGTT107150.08.9527831
CGGGCGT143250.08.9370345
AGGGGCG89350.08.74531651
GGCGAAA76250.08.6162552
GTAGTCC544100.08.55175826
TACTCGG307150.08.54863837
AGCCGGG381400.08.429992
CTACTCG319900.08.3274936
Produced by FastQC (version 0.11.5)