FastQCFastQC Report
Thu 8 Feb 2018
YD5_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD5_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64776131
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG145250.010.6089381
TAGGCCG38800.09.8707271
TGGCCGG151900.09.0469881
AGGGTTT368650.08.744951
TGGGGTT390500.08.6883211
TGGGGCG109700.08.5226251
AGGGGCG125550.08.48743251
TGGCCAG676150.08.3130311
TGGGCCG101550.08.2748631
AGGCGCC163450.08.2285131
TAGCCAG434550.08.1704951
TGGGGCC337300.08.1551371
AGGGCCG107600.08.1445911
TGGCAAA359850.07.96338131
GGGGTTT425800.07.8625422
AGGGGCC281000.07.77673531
TGGCGTT52800.07.68016431
TGGGGGC354600.07.64926431
AGGGGGG142000.07.6311271
TGGGCGG129650.07.5239741
Produced by FastQC (version 0.11.5)