FastQCFastQC Report
Thu 8 Feb 2018
YD7_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD7_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62496482
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG119950.010.7577331
TGGGGTT376500.09.4864691
AGGCGTG241850.09.1718421
AGGGTTT347350.09.1026141
TGGGGCG118800.09.0231241
AGGGGGC266500.08.8643051
AGGGGCG130350.08.8628361
GGGGTTT400850.08.85738852
AGGCGCC171000.08.8104161
TGGGGCC335950.08.7612761
TGGGGGG278050.08.7228661
TAGGCCG34350.08.7194951
AGGGGCA312600.08.6880991
AGGGCGG143150.08.6838861
AGGGGGG149050.08.62721351
TGGGCCG106200.08.6093021
TGGGGGC371750.08.535451
AGGGGGT201150.08.4862451
AGGCCGG183950.08.4227721
TGGCCAG617450.08.4032471